产品名称:重组小鼠粒细胞集落刺激因子(G-CSF)

产品英文名称:Mouse G-CSF protein

货号:PRP1308

产品特点&优势:
通过LAL法测定,每微克蛋白里内毒素含量<1.0 EU。

重组小鼠粒细胞集落刺激因子(G-CSF)产品背景:
Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.

序列:
氨基酸序列来源于:重组小鼠粒细胞集落刺激因子(G-CSF) (P09920) (V31-A208) 表达的蛋白片段。

标签:无标签

活性:
通过其诱导M-NFS-60细胞增殖的能力来测定。ED50为2.038 pg/mL。

蛋白长度:
重组小鼠粒细胞集落刺激因子(G-CSF) 由178个氨基酸组成,预测分子量为18.95 KD。

分子:
G-CSF; Colony Stimulating Factor 3; Granulocyte; GCSF

检测指标:
G-CSF; Colony Stimulating Factor 3; Granulocyte; GCSF

产品形式:冻干粉,冻干缓冲液为无菌的20 mM Tris,150 mM NaCl,pH 7.4。

重组小鼠粒细胞集落刺激因子(G-CSF)产品注意事项:
开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 μg/mL。客户可根据后续的实验需求,进行进一步的稀释。

警告:
本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。