产品名称:重组小鼠高迁移率族蛋白1,His-标签(无动物源)(HMGB1)

产品英文名称:Mouse HMGB1 Protein, His tag (Animal-Free)

货号:PRP1306

产品特点&优势:
通过LAL法测定,每微克蛋白里内毒素含量<0.1 EU。

重组小鼠高迁移率族蛋白1,His-标签(无动物源)(HMGB1)产品背景:
High Mobility Group protein B1 protein (HMGB1) is the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 25 kDa single chain polypeptide containing three domains: two N-terminal HMG boxes A and B, and a negatively charged 30 a.a. C-terminal region that contains only Asp and Glu. Post-translational modification on HMGB1 have been reported, affects its localization, receptor interactions, and function. HMGB1, with a disulfide bond between C23 and C45 , have been reported that cause cytokine production and the activation of NF-κB. Otherwise, the fully oxidized form has no immune function, losing its proinflammatory effect and the apoptotic cell death activation function.

序列:
来源于小鼠HMGB1 (Met1-Glu215) (P63158)氨基酸序列在c端添加6×His标签。

标签:His Tag (C-term)

活性:
通过其在RAW264.7细胞中诱导TNF α的能力来测量。该效应的ED?0 <15 ng/mL。

蛋白长度:
重组小鼠 HMGB1 由215个氨基酸组成,预测分子量为25.56 kDa。

分子:
HMG-1, HMG1, HMG3, SBP-1

检测指标:
HMG-1, HMG1, HMG3, SBP-1

产品形式:冻干粉,冻干缓冲液为无菌的1X PBS,pH 7.4。

重组小鼠高迁移率族蛋白1,His-标签(无动物源)(HMGB1)产品注意事项:
开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 μg/mL。客户可根据后续的实验需求,进行进一步的稀释。

警告:
本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。